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Project of Client-based VPN Remote Access Solution for the worldwide Retail Business Company in the region of Central Europe
Ondrák, Richard ; Havlín, Petr (referee) ; Kříž, Jiří (advisor)
The aim of this Thesis is to project and describe new Client-based Remote Access Solution for the Tesco Stores retail company in the region of Central Europe. Based on facts that the all present Tesco’s Remote Access Systems have not any longer met the increasing requirements and needs of corporate users and suppliers it is clear that the main scope is to find and implement such Solution that will meet and exceed all requirements. The main Project Aspects are to be the ease of use, security of the solution, unexacting administration of the system and user groups, and one unified Solution for the all Countries from the region of Central Europe that is suitably integrated in the Tesco IT infrastructure.
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Implementation of wall functions into a hybrid fictitious domain-immersed boundary method
Kubíčková, Lucie ; Isoz, Martin
Hybrid fictitious domain-immersed boundary method (HFDIB) is a simulation approach used in computational fluid dynamics. The approach avoids usage of complex geometry-conforming computational domains. Instead, a simple domain is used and the geometry is projected onto it by a scalar field and adjustment of governing equations. Hence, the time spent on mesh generation is substantially reduced. It is advantageous to use the HFDIB in geometry optimizations where it allows for a massive optimization speed-up. Nevertheless, there is a problem with simulation of the fluid behavior in the boundary layer in the vicinity of the immersed walls. Especially, in simulation of highly turbulent flows, where the boundary layer is very thin and the usage of finer mesh is unaffordable. In this work, we aim to solve this problem by implementation of Reynolds averaged turbulence models in our custom HFDIB variant. In particular, we implemented the k-ω turbulence model and blended wall functions for closure variables and velocity.
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On Reynolds-averaged turbulence modeling with immersed boundary method
Kubíčková, Lucie ; Isoz, Martin
The immersed boundary (IB) method is an approach in the computational fluid dynamics in which complex geometry conforming meshes are replaced by simple ones and the true simulated geometry is projected onto the simple mesh by a scalar field and adjustment of governing equations. Such an approach is particularly advantageous in topology optimizations (TO) where it allows for substantial speed-up since a single mesh can be used for all the tested topologies. In our previous work, we linked our custom IB variant, the hybrid fictitious domain-immersed boundary method (HFDIB), with a TO framework and successfully carried out an optimization under laminar flow conditions. However, to allow for optimizations of reallife components, the IB approach needs to be coupled with an affordable turbulence modeling. In this contribution, we focus on extending the HFDIB approach by the possibility to perform Reynolds-averaged simulations (RAS). In particular, we implemented the k − ω turbulence model and wall functions for closure variables and velocity.
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Odchov násady kapra v intenzivní akvakultuře
KOLAŘÍK, Filip
The common carp (Cyprinus carpio, L.) is a typical pond fish. However, intensive rearing systems aimed at rearing this fish can also be found in production aquaculture. Mainly for the rearing of two-year carp in winter. Such systems have been operated, for example, in Poland and Germany. In Poland, cage farms located on canals supplied with warmed water from the thermal power stations (Dolna Odra, Konin-Goslawice) were used. In Germany, due to the high losses on carp fry and two-year carp caused by cormorants during wintering, a similar rearing method was adopted. Breeding in flow-through systems (Schwarze pumpe, Jänschwalde) and recirculation systems (Neiden) were used. The aim of this bachelor's thesis was to evaluate and compare intensive carp broodstock rearing abroad and semi-intensive carp broodstock rearing used in the Czech Republic. At the same time, the bachelor thesis included an experiment in a recirculation system in Blatná, focused on winter rearing of two-year carp in the first year of the breeding cycle. The experiment was carried out in the recirculation system of the company Blatenská ryba s.r.o. The experiment lasted for 227 days, from the 1st of October 2021 to the 16th of May 2022. The first phase lasted from the 1st of October 2021 to the 8th of February 2022 and was accompanied by low growth and the development of lordosis. At the beginning of the phase, 6 800 carp fry were stocked into a rearing tank with a volume of 9.5 m3. The average weight of the stocked fry was 11,54 g.pcs-1. At the end of the first phase, the carp reached a weight of 50 g.pcs-1. The second phase of the experiment was conducted from February 8th, 2022 to May 16th, 2022. The phase was focused on a feeding experiment. The carp were divided into three tanks of 1,575 pieces each. Each tank was fed a different type of feed. At the end of the experiment, the best results were achieved by the fish fed with the Aller aqua Classic+Master feed type. These fish achieved a weight of 210.57 g. pcs-1 during the rearing period. However, the feed AG SV was the best economically. The worst growth and economic results were achieved in the group fed with the Skretting carpe F feeding type. Losses over the experimental period were only a few tenths of a percent. However, the whole breeding was accompanied by problems with the occurrence of lordosis in the reared fish. The occurrence of lordosis was caused by the poor composition of the feed used in the first phase of the experiment. In particular, the lack of easily digestible phosphorus for the carp. The results show that by using winter rearing it is possible to shorten the normal rearing cycle by one to two years. Compared to semi-intensive pond rearing, minimal losses are achieved. However, more emphasis must be placed on fish nutrition and the quality of the stocked fry.
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Influence of feeding management on intensive breeding of pikeperch (Sander lucoperca)
KUČERA, Václav
This thesis is aimed on problematics of intensive pikeperch aquaculture and the influence of the feeding by different types of pellets and also different frequencies of feeding during the day. Whole thesis consists of two separate experiments. In the first experiment I tried to evaluate the influence of sinking and floating type of pellets on the growth abilities and production markers of juvenile pikeperch. First experiment was stocked with 8000 pcs of juvenile pikeperch (average weight 21,38g). These fish were kept in controlled conditions for 16 weeks (average temperature of 21,37 ? 0,66 °C (7:00) and 20,77 ? 0,55 °C (15:00), average dissolved oxygen levels of 127,43 ? 2,37 % (7:00) and 117,03 ? 1,14% (15:00) °C, 120 % and initial biomass of 13,42 kg/m?). These fish were fed by belt feeders two types of pellets (floating Skretting Europa and sinking Biomar Effico Sigma) with the same nutrient composition. After 16 weeks all the production markers (SGR, FCR, WG, TL, CF and survival) were examined. Almost all the parameters showed the statistically significant difference in favor of the sinking pellets. Although growth heterogenity turned out to be lower in the group fed by floating pellets. In the second experiment 600 individuals of juvenile pikeperch with average weight of 37,49 g were stocked in tanks. These fish were kept in controlled conditions for 6 weeks (average temperature of 21,37 ? 0,66 °C (7:00) and 20,96 ? 0,66 °C (15:00), average dissolved oxygen levels of 127,43 ? 2,37 % (7:00) and 117,03 ? 1,14 % (15:00) with initial biomass of 13,35 kg/m?). These fish were fed by automatic feeder Imetronic Self Feeder in 3 different frequencies a day (3,6 or 12 batches). Pellets Skretting Europa- 15F were used in this experiment. After 6 weeks of rearing production markers were examined. Growth heterogenity, somatic indexes, and fin erosion were also examined. Also, blood samples were taken. After data analysis no statistically significant difference was found among the production markers in all three groups. Growth heterogenity was proven to be the lowest in the group fed 3x a day and highest in the group fed 12x a day. Organosomatic indexes showed statistically significant difference of HSI (hepatosomatic index). Highest index was observed in group fed 3x a day. During the analysis of the data from biochemical analysis increased levels of albumins and tryglicerids were observed especially in the group fed 12x a day. Simultaneously, statistically significant differences among the amonia levels (lowest concentration in the group fed 3x a day and highest in group fed 12x a day) and glucose (lowest concentration in the group fed 3x a day, two other groups had almost the same level of glucose) levels in plasma were found. As a group with the best results was determined a group fed 6x a day. This group reached satisfying results in production markers (better than group fed 3x a day but slightly worse than group fed 12x a day). Also, lower growth heterogenity than in group fed 12x a day was observed. Group fed 6x a day also displayed the best working lipid metabolism in contrary to the group fed 12x a day which displayed the disorder of the lipid metabolism. Also, the fin erosion was slightly lower than in a group fed 12x a day.
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Porovnání populací okouna říčního (Perca fluviatilis L.) z intenzivní akvakultury - vliv původu ryb na chování a kritickou rychlost plavání.
TOMÁŠEK, Ondřej
European perch is one of potential fish for breeding in conditions of intensive aquaculture. This species of fish is in relatively high demand, especially in the Alpine region, because of its very tasty meat. The relatively high price of fillets is attractive for producers. The difficulties in perch farming in conditions of European intensive aquaculture may alleviate the further domestication process of this species or its selective breeding. These pitfalls are negatively correlated with fish welfare. In this thesis, we examined morphometric (morphometry, fin damage, somatic index), behavioural (activity, aggressivity, boldness and critical swimming speed) and physiological (cortisol, glucose, enzyme activity, potassium) parameters in four populations of perch imported from different European intensive farms (France - two farms, Hungary, Denmark) engaged in their producing. Experiments performed in this thesis showed that individuals from the Danish population has the highest activity of the studied populations, including the critical swimming speed (4.24 ? 0.15 BL.sec-1). The population from Denmark also found the highest percentage (8.9 %) of bold exploratory behaviour, which subsequently increased aggressive behaviour within this population. The number of attacks on other individuals from the flock reached the highest measured values in this experiment (2.33 ? 2.31 attacks). Furthermore, experiments showed that individuals from the Danish population showed a higher tolerance for adverse environmental problems compared to other populations from Hungary or France. These differences, which have been observed in the Danish population, can be interpreted as a proactive stress mechanism that is characteristic of fast pace syndrome (fast POLS). High perivisceral fat index was found in fish from the Hungary. The morphometric characteristics of all populations were significantly different. Genomic characteristics could address this approach in the near future, in particular to streamline domestication processes and support the selective breeding of perch suitable for European intensive aquaculture.
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Vliv nasycení kyslíku ve vodě na efektivitu intenzivního chovu candáta obecného (Sander lucioperca) v recirkulačním akvakulturním systému
VOJTA, Petr
This thesis deals with an issue of Zander (Sander lucioperca) production in a recirculating aquaculture system RAS in different oxygen rates (50-80% O-, 80-110% Os and O+ 110-150%). Zander weighted W=22,98?15g was planted into two identical recirculating systems with different water temperature. SGR, FCR and FC of survival and general health condition of kept fish in hematology, biochemistry as well as stress indicators were evaluated. The results proved a negative influence of hypoxia (50-80%) on the food reception, growth and general survival of experimental fish kept in this environment in both the recirculating systems with water temperature 20 and 23°C. In the group O- kept in RAS 20°C, higher coefficient of FCR=1,11 was proved in comparison with group O (normoxia) and group O+ (hyperoxia), where no significant difference (p0,05) was recorded. The health condition showed a significant difference (p0,05) of O group in comparison with group O+ and O in RAS 20°C during the TBARS evaluation (brain, gills, muscle), assessing SOD in liver, and GPx (brain and muscle). By determining the HSI, SSI and VSI indexes, the significant differences (p0,05) of HSI and VSI for O+ and O groups in comparison with O- were proved.
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Vliv hustoty obsádek sumečka afrického (Clarias gariepinus) na efektivitu jeho intenzivního chovu v recirkulačním akvakulturním systému
TRNKA, Kamil
This study is a dressing the breeding process of the african catfish kept in RAS recycling waste heat from the biogas plant. The weight, SGR, FCR, FC, survival rate and were evaluated. During the first test different breeding densities were established (30, 60, 90, 120 kg.m-3) and fishes were kept in the tanks without lowering the the density to the original. Into the tanks with the same quality of the water, we introduced juveniles of the african catfish. Results of the first test showed that the best growth of the african catfish is achieved in the breeding density up to 230 kg.m-3. In the second experiment, the different breeding densities were established (90, 120, 150 a 180 kg.m-3). These densities were periodically lowered to the starting density each Month. In this test we achieved higher SGR and lower FCR. There were no significant differences among the density's groups. Third test was based on the presence of the sorting of the fish in tanks. While not sorting negative influence has appeared. In the non-sorted group, we found couple of marked-sized fish and couple of bellow average fish. Also, the growth parameters showed better results in the sorted group. All experiments showed that the best breeding density is from 180 up to 230 kg.m-3.
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Zhodnocení odpadu z akvakultury
LUNDA, Roman
Water treatment technology has undergone a dynamic development in recent years with new treatment methods rapidly emerging. Constructed wetlands, aerobic composting, anaerobic treatment, enzymatic or chemical hydrolysis, and aquaponics are conventional and well-known technologies used in aquaculture waste reduction, valorisation, and recycling. All aquaculture waste can be valorised by several technologies. Novel technologies are mainly applied to recycle resources or produce valuable by-products, including biodiesel, fish silage, biochar, lactic acid, hydrogen, and insect larvae growth from aquaculture sludge, as well as phytoremediation and biofloc technology for aquaculture sludge treatment. Chapter 2 documented the composition of sludge and wastewater from several RASs. The results show a possibility of wastewater utilization for plant production in the form of aquaponic or hydroponic systems. The benefits of aquaponics relate not only to the efficient uses of land, water and nutrient resources, but also allow for the increased integration of smart energy opportunities such as biogas and solar power. In this regard, aquaponics is a promising technology for producing both high-quality fish protein and vegetables in ways that can use substantially less land, less energy and less water - while also minimizing chemical and fertilizer inputs that are used in conventional food production. Chapter 2 also evaluated nutrient concentration according to RAS adjustment and the problem of high sodium concentrations. There is no perfect composition of sludge or wastewater for the requirements of plants. But its nutrient value can reduce costs for hydroponics nutrient solution production. Creating valuable fertilizer matter by vermicomposting represents a very promising technique for the future. This technology does not require unnecessarily high costs and its environment friendly. Chapter 3 documents aquaculture sludge composition and utilization for earthworm vermiculture. This chapter also includes toxicity and other possibilities for aquaculture sludge utilization as direct field application. This gives sludge from standard freshwater RAS a great opportunity and opens the doors for its direct use in the vermicomposting sector. It is well known that aquaculture solids treatment can be profitable thanks to biofloc technology. This technology presents a pillar in aquaculture waste valorisation. Leveraging nutrient rich biomass from biofloc technology as a potential feed source was presented in Chapter 4. It has been proven that almost 33% of feed could be replaced by biofloc meal for crayfish culture. The biggest problems of aquaculture waste valorisation by plenty of possible technologies is its variability. To achieve the ideal composition, it is necessary to choose the right approach in the aquaculture system. However, further research and developments are needed, as demonstrated by the challenges described in this thesis.
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The comparison of properties of cell lines resistant to ellipticine, doxorubicin, and cisplatin
Černá, Tereza ; Poljaková, Jitka (advisor) ; Eckschlager, Tomáš (referee)
7 Abstract Neuroblastoma is the most common extracranial solid tumor of childhood. Despite advances in cancer diagnosis and therapy, the treatment of some forms of neuroblastoma is still complicated. One of the major complications of the chemotherapy is a developed drug resistance. This master thesis deals with the effect of cytostatics on protein and gene expression of selected proteins, which may contribute to chemoresistance of the human neuroblastoma cell line UKF-NB-4. The sensitive line UKF-NB-4 and the resistant line UKF-NB-4CDDP , UKF-NB-4DOXO and UKF-NB-4ELLI were exposed to cisplatin, doxorubicin, ellipticine for 24, 48 and 72 hours. The Western blot analysis showed that cytostatic agents cisplatin, doxorubicin or ellipticine added to the sensitive neuroblastoma cell line UKF-NB-4 in amounts which are added to resistant neuroblastoma cell lines in order to maintain resistance induced expression of p53 and reduced expression of retinoblastoma protein pRb after 72 hours of cultivation. Differences in the expression of RAS protein, cytochrome P450 1A1, 3A4 and cytochrome b5 has not been shown. Changes in the expression of the studied proteins in resistant lines UKF-NB-4CDDP , UKF-NB-4DOXO and UKF-NB-4ELLI cultured with and without cytostatic agents were not detected by the Western blot analysis....
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